The use of fluorescently tagged antibodies to detect specific antigens dates as far back as 1942, when Coons et. al. published a paper entitled “Demonstration of pneumoccocal antigen in tissues by use of fluorescent antibody” in the Journal of Immunology. In it, the authors describe experiments in which concentrated anti-pneumococcal rabbit serum is labeled with fluorescein-4-isocyanate and used to stain various tissues from mice that have been infected with pneumococcus 3. This technique, now usually referred to as immunofluorescence staining or simply immunofluorescence, has been modified some in the sixty years since it was first introduced, but it is still widely used today. Because of this, there are a number of companies involved in antibody production – most of these companies offer a suite of services that allow researchers a broad choice of production packages. Take a look below at a few of these antibody production companies.

Sigma-Genosys

Sigma-Genosys provides a complete service for polyclonal antibody production. The complete antisera service includes high quality custom peptide synthesis, conjugation, immunization, and sera collection.
We synthesize approximately 10mg of peptide for your antibody project. The peptide is analyzed by mass spectral and HPLC analyses to ensure sequence integrity and purity prior to conjugation. Next, the peptide is conjugated to KLH or BSA (2-3mg). The peptide-protein conjugate is purified by gel filtration and freeze dried. The remaining unconjugated peptide is supplied to the customer
Peptide Antisera