2006-08-18 - The assessment of cell proliferation and viability of cells in culture is a cornerstone for cancer biologists. Cytotoxicity assays provide valuable screening methods for novel anti-cancer drugs, apoptotic agents and cytostatic compounds. There are a number of methods available to test cell viability ... more

2006-08-21 - The Protean® IEF Cell is a convenient apparatus for quick separation of proteins based on isoelectric point (pI). As compared to the conventional methods of separation, which take approximately 20 hours, Bio-Rad’s Protean® IEF Cell can complete the run in 5 hours, which accelerates research. It ... more

2004-11-23 - The DAKO® Fuchsin Substrate-Chromogen System (catalog #K0624) is intended for use in immunohistochemical (IHC) and in situ hybridization staining procedures where alkaline phosphatase is used as the enzyme marker. The Fuchsin substrate-chromogen yields a fuchsia-colored reaction product at the site ... more

2001-02-16 - Researchers interested in using genetic reporters to study gene expression should consider using the Promega Luciferase Assay System. This popular kit is relatively quick, easy to use and involves little hands-on time. In general, firefly luciferase is extensively used as a reporter because one only ... more

2004-10-21 - The SignalStain™Phospho-p44/42 (Thr202/Tyr204) IHC Detection Kit from Cell Signaling Technology (#8110) is a detection method for the activation of MAP kinase in human tissue and cell preparates by immunohistochemistry (IHC). The kit, used to determine cell growth/proliferation, uses an immunopero ... more

2001-07-18 - One of the problems you always run into while performing immunofluorescence staining is photobleaching or “fading”. This problem can limit the number of images you are able to get from one sample and hence can seriously affect your results. To deal with this problem, Molecular Probes has develop ... more

2000-11-01 - Enzyme-Labeled Fluorescence 97 (ELF-97) is a new substrate developed by Molecular Probes for alkaline phosphatase (AP) which is widely used as a reporter gene in various applications. ELF-97 is a soluble, weakly blue-fluorescent, phosphorylated molecule that yields a bright yellow-green fluorescent ... more

2005-10-25 - Although the standard protocol for the purification of antibodies from serum using either recombinant Protein G or Protein A cross-linked to agarose beads is a proven technique, the method is arguably laborious and time-consuming. Furthermore, both Protein A and G have varying affinities for differe ... more

2006-07-06 - The BD FACSCanto™ is often hailed as the new generation of flow cytometry. One striking difference between the FACSCanto™ and older generation machines such as the FACSCalibur™ is that this system operates on a PC-based Windows XP® platform using BD FACSDiva™ software. These tools assist in ... more

2001-04-18 - When FlowJo 2.1 was first released in June 1997 it generated considerable interest in our laboratory. For some time we had been frustrated with the applications available for analyzing our flow cytometric data and FlowJo promised to overcome many of those frustrations. Three years, several program r ... more